EBSGFV-9 15? kb1-GF2-GF3-GF5-GF1-GF4-GF15? kb1-IM2-IM

Dotted lines correspond to restriction-enzyme internet sites of BamH1/Hind3A on eBSOLV (A) and of BamH1/Spe1 on 6/61), II (9.1 , 3/33), Ia (7.9 , 3/38), V (7.0 , 5/71), VIII (5.9 , 1/17), IV (0 , 0/4), and VII (0 , 0/1). Capsular serotype, clonal complex eBSGFV (B) and eBSIMV (C). The size on the expected fragments is indicated in base pairs using fragment coding to get a, B and C.grouping accessions absent in the prior evaluation carried out by Gayral et al.EBSGFV-9 15? kb1-GF2-GF3-GF5-GF1-GF4-GF15? kb1-IM2-IM 3-IM 4-IM 7-IM5-IM2-IM 3-IM 4-IM6-IM1KBPositive r 0 n j de K i2 i3 nd ont am i1 t n 16 26 3 -8 Control La Ma Lad Or 6 LC Ch Ch Ch Ba ten 16 63 Ho M C BB PKW BB BB BB BB BB BB OG OG OG OG BB BB BB BB BB1KBPositive Handle BB PKW16 BB -8 0 n j i3 nd ont am CK hi1 hi2 n t Or La Ma C C Ch Ba M C L BB OG OG BB BB BB BB BB OG OG1KBDE2-GF/3761 bp 3-GF/3134 bp 1-GF/3030 bp 4-GF/2888 bpBB6 BB3 BBBBHoF1-IM/4329bp 6-IM/3879 bpPositive Handle BB PKWBB16 BB6 BB0 n j i1 K i2 nd ont am i3 t n -8 three Ba La Ma Or M Ch Ch 16 63 LC C Ho Ch BB OG OG BB BB BB BB BB BB OG OG BB7-OL/8500 bp 9-OL/7516 bp 8-OL/5370 bp 6-OL+1-OL/4773 bp 4-OL/4181 bp 1-OL/3615 bp 2-OL/3335 bp 3-OL/2921 bp 3054 bp 7126 bp 6108 bp 5090 bp5-IM/2918 bp 4072 bp 5-GF/2299 bp5-OL/2351 bp 2036 bp1636 bp 3-IM/1478 bp6-OL/1158 bp 1018 bp 3'-GF/640 bp2-IM/1131 bp 7-IM/1065 bp 4-IM/977 bpFIG.EBSGFV-9 15? kb1-GF2-GF3-GF5-GF1-GF4-GF15? kb1-IM2-IM 3-IM 4-IM 7-IM5-IM2-IM 3-IM 4-IM6-IM1KBPositive r 0 n j de K i2 i3 nd ont am i1 t n 16 26 3 -8 Control La Ma Lad Or six LC Ch Ch Ch Ba ten 16 63 Ho M C BB PKW BB BB BB BB BB BB OG OG OG OG BB BB BB BB BB1KBPositive Manage BB PKW16 BB -8 0 n j i3 nd ont am CK hi1 hi2 n t Or La Ma C C Ch Ba M C L BB OG OG BB BB BB BB BB OG OG1KBDE2-GF/3761 bp 3-GF/3134 bp 1-GF/3030 bp 4-GF/2888 bpBB6 BB3 BBBBHoF1-IM/4329bp 6-IM/3879 bpPositive Manage BB PKWBB16 BB6 BB0 n j i1 K i2 nd ont am i3 t n -8 three Ba La Ma Or M Ch Ch 16 63 LC C Ho Ch BB OG OG BB BB BB BB BB BB OG OG BB7-OL/8500 bp 9-OL/7516 bp 8-OL/5370 bp 6-OL+1-OL/4773 bp 4-OL/4181 bp 1-OL/3615 bp 2-OL/3335 bp 3-OL/2921 bp 3054 bp 7126 bp 6108 bp 5090 bp5-IM/2918 bp 4072 bp 5-GF/2299 bp5-OL/2351 bp 2036 bp1636 bp 3-IM/1478 bp6-OL/1158 bp 1018 bp 3'-GF/640 bp2-IM/1131 bp 7-IM/1065 bp 4-IM/977 bpFIG. 2. eBSV Southern blot evaluation of Musa diploids. (A ) In silico restriction-enzyme fragments of PKW eBSVs. Dotted lines correspond to restriction-enzyme internet sites of BamH1/Hind3A on eBSOLV (A) and of BamH1/Spe1 on eBSGFV (B) and eBSIMV (C). Grey, dark grey and black boxes represent a one of a kind fragment existing within the eBSV allele, a fragment repeated inside the eBSV allele plus a distinct eBSV allelic fragment, respectively. The 9-OL fragment (A) corresponds to non-digestion at the restriction web page within the 2-OL and 4-OL fragments, the 6-OL?-OL fragment (D) corresponds to partial digestion and is always associated with all the 6-OL and 1-OL fragments, as well as the 3-GF fragment (B) represents the two fragments 30 -GF and 300 -GF observed around the Southern blot pattern (E). (D ) Southern blot patterns for any set of banana DNA hybridized with BSOLV, BSGFV and BSIMV probes, respectively. Digested DNA of M. balbisiana diploid accessions (in red) and out-group accessions (in black), noted according 369158 to Table 1. These Southern blots had been hybridized together with the full-length BSOLV (D), BSGFV (E) and BSIMV (F) ijerph7041855 probes. The initial lane on left for each and every Southern blot would be the reference PKW pattern. The right-most lane (D only) may be the 1-kb ladder (Invitrogen, Life Technology). The size of your expected fragments is indicated in base pairs making use of fragment coding for any, B and C.grouping accessions absent in the preceding analysis accomplished by Gayral et al. (2010).