0.15 P=0.04 P=0.43 siLUC UPF1 Phosphorylation levels (fold induction) 4 three.5 3 2.5 2 1.5 1 0.five 0 0 2 Time (h) four P

Indicated values are normalized T enriched preferably in clusters with strong centromeric influences (Fig. 5c towards the zero jmir.6472 time point .e.m. P value is comparing siLUC to siZFP36/L1/L2 situations at 1 h right after serum induction making use of the paired two-tailed Student's t-test.NATURE COMMUNICATIONS | 7:12434 | DOI: 10.1038/ncomms12434 | www.nature.com/naturecommunicationsNATURE COMMUNICATIONS | DOI: ten.1038/ncommsARTICLEUPFCa1 two 34 5 S42 T44 S97 S10* T28*CHHelicase6 7 8 S1031 S1038 S9 ten 11 S1046 S1050 T12 13 14 S1062 S1065 S15 16 17 18 S1073* S1078* S1089 S1096*19 S1116* 1397 nts 818 nts 1397 nts 818 nts 1397 nts 818 ntsb ExogenousUPF1: Chase (min): WT GAP 0 None 120 240 360 0 UPF1-C126S 120 240 360 0 UPF1-wt 120 240 360UPF1 [S/T] 7,8,19 A 120 240UPF1 [S/T] 7,eight,9,10,11,19 A 0 120 24039 t1/2 (min) Exogenous UPF1 : Chase (min): WT GAP 0 610' ?4 UPF1 [S/T] 15,16 A 120 240 360 0 592' ?9 UPF1 [S/T] 1,two A 120 240 360 0 137' ?9 UPF1 [S/T] 1,two,15,16 A 120 240 360 0 148' ?six UPF1 [S/T] 17,18 A 120 240 360 0 165' ?2 UPF1 [S/T] 7,8,9,10,11, 17,18,19 A 120 24039 t1/2 (min) 156' ?four 117' ?1 155' ? UPF1 [S/T] 1,2,9,10,11 15,16,17,18 A 0 120 240 360 260' ?1 UPF1 [S/T] 7,eight,9,10,11 15,16,17,18,19 A 0 120 240 360 368' ?5 UPF1 [S/T] 1,two,7,eight,9,ten,11, 15,16,17,18,19 A 0 120 240Exogenous UPF1 : Chase (min): WT GAPUPF1 [S/T] 15,16,17,18 A 120 240UPF1 [S/T] 1,two,15,16,17,18 A 0 120 24039 t1/2 (min) 492' ?eight 585' ?three 658' ?9 619' ?7 631' ?c39 mRNA half life (min)800 700 600 500 400 300 200 one hundred UPF1-wt UPF1-[S/T] 7,8,19 A UPF1-[S/T] 7,eight,9,10,11,19 A UPF1-[S/T] 15,16 A UPF1-[S/T] 1,2,15,16 A UPF1-[S/T] 17,18 A UPF1-[S/T] 15,16, 17,18 A UPF1-[S/T] 1,2, 15,16,17,18,A UPF1-[S/T] 1,two,9,ten,11 15,16,17,18, A UPF1-[S/T] 7,eight,9,ten,11 15,16,17,18,19 A UPF1-[S/T] 1,2 A UPF1-[S/T] 7,8,9,10,11, 17,18,19 A UPF1-[S/T] 1,two,7,eight,9,ten 11,15,16,17,18,19 A UPF1-C126S 17470919.2015.1029593 None* ** * ******0 Exogenous UPF1:Figure 4 | A number of UPF1 [S/T]Q motifs contribute to NMD. (a) Schematic representation of human UPF1 protein displaying the amino acids mutated within this study (black); the position of the helicase domain as well as the cysteine/histidine-rich domain (CH) of UPF1 are indicated (to not scale). Numbers following amino-acid symbols correspond towards the position in the UPF1 principal sequence (GenBank #NP_002902). Asterisk indicates confirmed phosphorylated websites. [S/T]Q motifs not investigated in this perform are shown in grey.